biocompatibility of genipin and glutaraldehyde cross-linked chitosan materials in the anterior chamber of the eyegenipin和戊二醛交联壳聚糖材料的生物相容性前房的眼睛.pdf

Int. J. Mol. Sci. 2012, 13, 10970-10985; doi:10.3390/ijms130910970 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Biocompatibility of Genipin and Glutaraldehyde Cross-Linked Chitosan Materials in the Anterior Chamber of the Eye Jui-Yang Lai 1,2,3 1 Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan; E-Mail: jylai@.tw; Tel.: +886-3-211-8800 (ext. 3598); Fax: +886-3-211-8668 2 Biomedical Engineering Research Center, Chang Gung University, Taoyuan 33302, Taiwan 3 Molecular Medicine Research Center, Chang Gung University, Taoyuan 33302, Taiwan Received: 4 June 2012; in revised form: 2 August 2012 / Accepted: 27 August 2012 / Published: 4 September 2012 Abstract: Chitosan is a naturally occurring cationic polysaccharide and has attracted much attention in the past decade as an important ophthalmic biomaterial. We recently demonstrated that the genipin (GP) cross-linked chitosan is compatible with human retinal pigment epithelial cells. The present work aims to further investigate the in vivo biocompatibility of GP-treated chitosan (GP-chi group) by adopting the anterior chamber of a rabbit eye model. The glutaraldehyde (GTA) cross-linked samples (GTA-chi group) were used for comparison. The 7-mm-diameter membrane implants made from either no