biocompatibility and alkaline phosphatase activity of phosphorylated chitooligosaccharides on the osteosarcoma mg63 cell line生物相容性和磷酸化的碱性磷酸酶活性低聚糖在骨肉瘤mg63细胞线.pdf

J. Funct. Biomater. 2010, 1, 3-13; doi:10.3390/jfb1010003 OPEN ACCESS Journal of Functional Biomaterials ISSN 2079-4983 /journal/jfb/ Article Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line 1 1 2 1 Jayachandran Venkatesan , Ratih Pangestuti , Zhong-Ji Qian , BoMi Ryu and Se-Kwon Kim 1,2,* 1 Department of Chemistry, Pukyong National University, Busan 608-737, Korea; E-Mails: venkatjchem@ (J.V.); pangesturth@ (R.P.); ryu.bomi@ (B.R.) 2 Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea; E-Mail: qianzhongji@ (Z.Q.) * Author to whom correspondence should be addressed; E-Mail: sknkim@pknu.ac.kr; Tel.: +82-51-629-7097; Fax: +82-51-628-8147. Received: 26 August 2010; in revised form: 7 October 2010 / Accepted: 20 October 2010 / Published: 22 October 2010 Abstract: Phosphorylated chitooligosaccharides (P-COS) were prepared using a H PO , 3 4 P O , Et PO and hexanol solvent system. The P-COS were characterized by Fourier 2 5 3 4 Transform Infrared Spectroscopy (FT-IR), T