streptozotocin-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in human hepatoma hepg2 cells体外细胞毒性,氧化应激和线粒体功能障碍在人类肝癌hepg2细胞.pdf

Int. J. Mol. Sci. 2012, 13, 5751-5767; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Streptozotocin-Induced Cytotoxicity, Oxidative Stress and Mitochondrial Dysfunction in Human Hepatoma HepG2 Cells Haider Raza * and Annie John Department of Biochemistry, Faculty of Medicine and Health Sciences, UAE University, POBox 17666, Al Ain, United Arab Emirates; E-Mail: anniej@uaeu.ac.ae * Author to whom correspondence should be addressed; E-Mail: h.raza@uaeu.ac.ae; Tel.: +971-3-7137506; Fax: +971-3-7672033. Received: 7 March 2012; in revised form: 12 April 2012 / Accepted: 26 April 2012 / Published: 11 May 2012 Abstract: Streptozotocin (STZ) is an antibiotic often used in the treatment of different types of cancers. It is also highly cytotoxic to the pancreatic beta-cells and therefore is commonly used to induce experimental type 1 diabetes in rodents. Resistance towards STZ-induced cytotoxicity in cancer cells has also been reported. Our previous studies have reported organ-specific toxicity and metabolic alterations in STZ-induced diabetic rats. STZ induces oxidative stress and metabolic complications. The precise molecular mechanism of STZ-induced toxicity in different tissues and carcinomas is, however, unclear. We have, therefo