cytotoxicity and transcriptional activation of stress genes in human liver carcinoma (hepg2) cells exposed to iprodione人类肝细胞毒性应激基因转录激活癌(hepg2细胞暴露于iprodione).pdf

Int. J. Environ. Res. Public Health 2004, 1, 12–20 International Journal of Environmental Research and Public Health ISSN 1660-4601 © 2004 by MDPI Cytotoxicity and Transcriptional Activation of Stress Genes in Human Liver Carcinoma (HepG2) Cells Exposed to Iprodione Teresa Washington 1 and Paul B. Tchounwou 1* 1Molecular Toxicology Research Laboratory, NIH-Center for Environmental Health, School of Science and Technology, Jackson State University, 1400 Lynch Street, P.O. Box 18540, Jackson, Mississippi, USA. * Correspondence to Dr. Paul B. Tchounwou. Email: paul.b.tchounwou@ Received: 11 September 2003 / Accepted: 13 November 2003 / Published: 29 February 2004 Abstract: Iprodione (C H Cl N O ) is a broad spectrum dicarboximide fungicide used on a wide variety of 13 13 2 3 3 crop diseases. It is used on vegetables, ornamentals, pome and stone fruit, root crops, cotton and sunflowers, to control a variety of fungal pests. Iprodione inhibits the germination of spores and the growth of the fungal mycelium. Experimental studies with mice have indicated that exposure to iprodione at dose levels 5 to 15 folds greater than the LOAEL for liver injury, induces microsomal enzyme activities, hepatocyte proliferation, hepatomegaly, centrilobular hypertrophy, diffuse hypertrophy, and an increase in lauric acid hydro