synthesis and effect on human hepg2 cells of 1,2-bis- (2-methylallyl)disulfane对人类hepg2细胞合成和影响,以叔u2014u2014disulfane(2-methylallyl).pdf

Molecules 2010, 15, 3634-3642; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis and Effect on Human HepG2 Cells of 1,2-bis- (2-Methylallyl)disulfane Chunxiao Ji 1,*, Fenglian Ren 1, Jun Dai 1 and Ming Xu 2 1 College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China; E-Mail: renfl2009@ (F.R.) 2 Research Institute for Molecular Pharmacology and Therapeutics, Central South University, Changsha, Hunan 410083, China  Author to whom correspondence should be addressed; E-Mail: cx312@163.com. Received: 16 April 2010; in revised form: 11 May 2010 / Accepted: 12 May 2010 / Published: 18 May 2010 Abstract: 1,2-bis(2-methylallyl)disulfane was synthesized from sodium sulfide and 3-chloro-2-methylpropylene. The structure of the target product was confirmed by GC-MS, 1H-NMR and elemental analysis. Cell viability assay, flow-cytometric analysis and protein expression results showed that 1,2-bis(2-methylallyl)disulfane could significantly inhibit the proliferation, and induce the apoptosis of human HepG2 cells. Key words: 1,2-bis(2-methylallyl)disulfane; identification; apoptosis 1. Introduction Garlic is a kind of liliaceae allium, and allicin is the general name for the main bioactive components of garlic, which is a mixture of a variety of allyl sulfides, including diallytrisulfide compounds (DATS), diallyl disulfide compounds (DADS) and many kinds of thioethers. All