synthesis and protective effect of scutellarein on focal cerebral ischemiareperfusion in rats合成和scutellarein焦脑ischemiareperfusion在大鼠的保护作用.pdf

Molecules 2012, 17, 10667-10674; doi:10.3390/molecules170910667 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis and Protective Effect of Scutellarein on Focal Cerebral Ischemia/Reperfusion in Rats Lihua Qian 1, Minzhe Shen 1, Hao Tang 1, Yuping Tang 1,*, Li Zhang 1, Yifan Fu 1, Qianping Shi 1 and Nian-Guang Li 1,2,* 1 Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China 2 Department of Medicinal Chemistry, Nanjing University of Chinese Medicine, Nanjing 210046, China * Authors to whom correspondence should be addressed; E-Mails: yupingtang@ (Y.T.); linianguang@ (N.-G.L.); Tel./Fax: +86-25-8581-1916 (Y.T.). Received: 27 June 2012; in revised form: 13 August 2012 / Accepted: 28 August 2012 / Published: 6 September 2012 Abstract: Scutellarein, the main metabolite of scutellarin in vivo, has relatively better solubility, bioavailability and bio-activity than scutellarin. However, compared with scutellarin, it is very difficult to obtain scutellarein from Nature. Therefore, the present study focused on establishing an efficient route for the synthesis of scutellarein by hydrolyzing scutellarin. Neurological deficit score and cerebral infarction volume with the administration of scutellarein were then used to compare its neuroprotective effects on focal cerebral ischemia/reperfusion in rats induced by middle cerebral artery occlusion (MCAO)