cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats保护作用吸附和isoproterenol-induced danshensu组合在大鼠心肌损伤.pdf

Cardioprotective Effect of Paeonol and Danshensu Combination on Isoproterenol-Induced Myocardial Injury in Rats 1. 1. 1. 1 2 1 1 Hua Li , Yan-Hua Xie , Qian Yang , Si-Wang Wang *, Bang-Le Zhang *, Jian-Bo Wang , Wei Cao , Lin- 1 1 1 1 1 1 Lin Bi , Ji-Yuan Sun , Shan Miao , Jing Hu , Xuan-Xuan Zhou , Peng-Cheng Qiu 1 Institute of Materia Medica, School of Pharmacy, The Fourth Military Medical University, Xi’an, People’s Republic of China, 2 Department of Pharmaceutics, School of Pharmacy, The Fourth Military Medical University, Xi’an, People’s Republic of China Abstract Background: Traditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizaeare are prescribed together for their putative cardioprotective effects in clinical practice. However, the rationale of the combined use remains unclear. The present study was designed to investigate the cardioprotective effects of paeonol and danshensu (representative active ingredient of Cortex Moutan and Radix Salviae Milthiorrhizae, respectively) on isoproterenol-induced myocardial infarction in rats and its underlying mechanisms. Methodology: Paeonol (80 mg kg21) and danshensu (160 mg kg21) were administered orally to Sprague Dawley rats in individual or in combination for 21 days. At the end of this period, rats were administered isoproterenol (85 mg kg21) subcutaneously to induce myocardial injury. After induction, rats were anaesthetized with pentobarbital sodium (35 mg kg21) to record electrocardiogram, then sacrificed and biochemical ass