the effect of treadmill training pre-exercise on glutamate receptor expression in rats after cerebral ischemia跑步机训练运动前的谷氨酸受体表达的影响在脑缺血后大鼠.pdf

Int. J. Mol. Sci. 2010, 11, 2658-2669; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article The Effect of Treadmill Training Pre-Exercise on Glutamate Receptor Expression in Rats after Cerebral Ischemia Feng Zhang 1,†, Jie Jia 1,†, Yi Wu 1,*, Yongshan Hu 1 and Yang Wang 2 1 Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai, China 2 Department of Anatomy and Embryology, Fudan University, Shanghai, China; E-Mail: zjk20019@126.com † These authors contributed equally to this work. * Author to whom correspondence should be addressed: E-Mail: doctorwuyi@126.com; Tel.: +86-21-528-878-20; Fax: +86-21-528-878-20. Received: 4 June 2010; in revised form: 2 July 2010 / Accepted: 2 July 2010 / Published: 7 July 2010 Abstract: Physical exercise has been demonstrated to be neuroprotective in both clinical and laboratory settings. However, the exact mechanism underlying this effect is unclear. Our study aimed to investigate whether pre-ischemic treadmill training could serve as a form of ischemic preconditioning in a rat model undergoing middle cerebral artery occlusion (MCAO). Thirty-six rats were divided into three groups: a sham control group, a non-exercise with operation group and an exercise with operation group. After treadmill training, ischemia was induced by occluding the MCA for 2 h, followed by reperfusion. Half of t