comparative effect of treadmill exercise on mature bdnf production in control versus stroke rats比较跑步机锻炼对成熟的脑源性神经营养因子的影响生产控制与中风的老鼠.pdf

Comparative Effect of Treadmill Exercise on Mature BDNF Production in Control versus Stroke Rats ´ 1,2 1,3 1,2 ´ 4 1,2 Aurore Quirie , Marie Hervieu , Philippe Garnier , Celine Demougeot , Claude Mossiat , Nathalie Bertrand2, Alain Martin1,2, Christine Marie1,2, Anne Prigent-Tessier1,2* ´ ´ ´ 1 Unite INSERM U1093 Cognition, Action et Plasticite Sensorimotrice, Dijon, France, 2 Universite de Bourgogne, Dijon, France, 3 Department of Neurology, University ´ ´ ´ Hospital, Dijon, France, 4 EA 4267 Fonctions et Dysfonctions Epitheliales, Faculte de Medecine-Pharmacie, Besancon, France Abstract Physical exercise constitutes an innovative strategy to treat deficits associated with stroke through the promotion of BDNF- dependent neuroplasticity. However, there is no consensus on the optimal intensity/duration of exercise. In addition, whether previous stroke changes the effect of exercise on the brain is not known. Therefore, the present study compared the effects of a clinically-relevant form of exercise on cerebral BDNF levels and localization in control versus stroke rats. For this purpose, treadmill exercise (0.3 m/s, 30 min/day, for 7 consecutive days) was started in rats with a cortical ischemic stroke after complete maturation of the lesion or in control rats. Sedentary rats were run in parallel. Mature and proBDNF levels were measured on the day following the last boot of exercise using Western blotting analysis. Total BDNF levels were simultaneously measured using ELISA tests. As compared to the