apoptotic effects of antilymphocyte globulins on human pro-inflammatory cd4+cd28？ t-cells凋亡的影响抗淋巴细胞球蛋白对人类炎性cd4 + cd28 t细胞.pdf

Apoptotic Effects of Antilymphocyte Globulins on Human Pro-inflammatory CD4+CD282 T-cells 1,2 3 4 1 1 Christina Duftner , Christian Dejaco , Paul Hengster , Klaudija Bijuklic , Michael Joannidis , 4 1 Raimund Margreiter , Michael Schirmer * 1 Department of Internal Medicine, Clinic of Internal Medicine I, Innsbruck Medical University, Innsbruck, Austria, 2 Department of Internal Medicine, General Hospital of Kufstein, Kufstein, Austria, 3 Department of Rheumatology, Medical University Graz, Graz, Austria, 4 Department of Operative Medicine, Clinic of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria Abstract + 2 Background: Pro-inflammatory, cytotoxic CD4 CD28 T-cells with known defects in apoptosis have been investigated as markers of premature immuno-senescence in various immune-mediated diseases. In this study we evaluated the influence of polyclonal antilymphocyte globulins (ATG-Fresenius, ATG-F) on CD4+CD282 T-cells in vivo and in vitro. Principal Findings: Surface and intracellular three colour fluorescence activated cell sorting analyses of peripheral blood mononuclear cells from 16 consecutive transplant recipients and short-term cell lines were performed. In vivo, peripheral levels of CD3+ + 2 CD4 CD28 T-cells decreased from 3.7 67.1% before to 060% six hours after ATG-F application (P = 0.043) in 5 ATG-F treated but not in 11 control patients (2.9 62.9% vs. 3.963.0%). In vitro, ATG-F induced apoptosis even in CD4+CD282 + + T-cells, which