curcumin enhances cytotoxic effects of bortezomib in human multiple myeloma h929 cells potential roles of nf-κbjnk姜黄素在血液病中的作用增强细胞毒性影响人类多发性骨髓瘤h929细胞nf-κbjnk的潜在作用.pdf

Int. J. Mol. Sci. 2012, 13, 4831-4838; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Communication Curcumin Enhances Cytotoxic Effects of Bortezomib in Human Multiple Myeloma H929 Cells: Potential Roles of NF-κB/JNK Qing-Xian Bai * and Xiao-Yan Zhang Department of Hematology, State Center of Bone Marrow Transplantation, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China; E-Mail: zhishui2004@163.com * Author to whom correspondence should be addressed; E-Mail: baiqingx@; Tel.: +86-29 Fax: +86-29 Received: 9 March 2012; in revised form: 31 March 2012 / Accepted: 11 April 2012 / Published: 16 April 2012 Abstract: Combined curcumin and PS-341 treatment has been reported to enhance cytotoxicity and minimize adverse effects through ERK and p38MAPK mechanisms in human multiple myeloma cells. However, whether JNK plays similar role in this process remains unclear. In the present study, we found combined treatment altered NF-κB p65 expressions and distributions in multiple myeloma H929 cells. Western blot analysis showed combined treatment inactivated NF-κB while activated JNK signaling. Pre-treatment with JNK inhibitor SP600125 could attenuate NF-κB inactivation and restored H929 cells’ survival. These results suggested that curcumin might enhance the cytotoxicity of PS-341