comparative study on the therapeutic potential of neurally differentiated stem cells in a mouse model of multiple sclerosis比较研究治疗潜在的神经分化的干细胞在小鼠模型的多发性硬化症.pdf

Comparative Study on the Therapeutic Potential of Neurally Differentiated Stem Cells in a Mouse Model of Multiple Sclerosis 1 1 1 2 2 Natalie L. Payne , Guizhi Sun , Daniella Herszfeld , Pollyanna A. Tat-Goh , Paul J. Verma , 3 3 3 1 1 Helena C. Parkington , Harold A. Coleman , Mary A. Tonta , Christopher Siatskas , Claude C. A. Bernard * 1 Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia, 2 Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia, 3 Department of Physiology, Monash University, Clayton, Victoria, Australia Abstract Background: Transplantation of neural stem cells (NSCs) is a promising novel approach to the treatment of neuroinflammatory diseases such as multiple sclerosis (MS). NSCs can be derived from primary central nervous system (CNS) tissue or obtained by neural differentiation of embryonic stem (ES) cells, the latter having the advantage of readily providing an unlimited number of cells for therapeutic purposes. Using a mouse model of MS, we evaluated the therapeutic potential of NSCs derived from ES cells by two different neural differentiation protocols that utilized adherent culture conditions and compared their effect to primary NSCs derived from the subventricular zone (SVZ). Methodology/Principal Findings: The proliferation and secretion of pro-inflammatory cytokines by antigen-stimulated splenocytes was reduced in the presence of SVZ-NSCs, while ES cell-derived NSCs exert