Potentiation of （-）-epigallocatechin-3-gaUate-induced apoptosis by bortezomib. in multiple myeloma cells.pdf

／ 一 ActaBiochimBiophysSinf2009)：1018—1026I@TheAuthor2009．PublishedbyABBSEditorialOfficeinassociationwithOxfordUniversityPressonbehalfof theInstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences，ChineseAcademyofSciences．DOI：10．10g3／abbsg『mp0g4． ABBS AdvanceAccessPublication10November2009 Potentiationof(一)-epigaUocatechin一3-gallate—inducedapoptosisbybortezomib inmultiplemyelomacells QingWang，JuanLi，JingliGu，BeihuiHuang，YingZhao，DongZheng，YanDing，andLijinZeng DepartmentofHematology，TheFirstAffiliatedHospital，SunYat—senUniversity，Guangzhou510080，China Correspondenceaddress．Tel：+86—208831：Fax：+86—20E—mail：luliyuan@tom．corn The green tea constituent， (一)一epigallocatechin一3一 numberofcellularprocessesand itse衔cacy in many gallate (EGCG)，haschemopreventiveandanticancer tun3ormodelsystems 3『，41．Themechanism underlying effects．Thisispartiallybecauseoftheselectiveabliity EGCG—mediatedapoptosisofcancercellsisincomple— ofEGCG to induceapoptosisanddeath incancercells telyunderstood．Suchanunderstanding，however，could withoutaffectingnormalcells．Inthepresentstudy，the provetheimportanceofdevelopingEGCG andrelated activityofEGCG againstthemyelomacellline．KM3． compoundsas single agentsor in combination with wasexamined．Ourresultsdemonstrated．forthefirst otherdrugsforthepreventionand／ortherapyofcancer． time．that the treatmentofthe KM 3 cellline with Thetranscriptionfactor．NF．KB．playsacriticalrolein EGCG inhibitscellproliferationand inducesapoptosis． theregulation ofgenesrelatedtoeel1surviva1．prolifer． andthereisasynergisticeffectwhenEGCG andborte． ation，andapoptosis[5，6]．Undernormalconditions， zomibarecombined．Furtherexperimentsshowed~that NF—KB ispresentinthecytoplasm asaninactivehetero． thiseffectinvolvestheNF．KB pathway．EGCG inhibits trimerconsistingofP50，P65，andIK