comparative expression profiling of distinct t cell subsets undergoing oxidative stress比较分析不同的t细胞表达子集进行氧化应激.pdf

Comparative Expression Profiling of Distinct T Cell Subsets Undergoing Oxidative Stress 1 2,3 2 1 Rudolf Lichtenfels , Dimitrios Mougiakakos , C. Christian Johansson , Sven P. Dressler , 1 2 1 Christian V. Recktenwald , Rolf Kiessling , Barbara Seliger * 1 Institute of Medical Immunology, Martin Luther University Halle-Wittenberg, Halle, Germany, 2 Department of Oncology and Pathology, Cancer Center Karolinska, Stockholm, Sweden, 3 Department of Internal Medicine 5, Hematology and Oncology, University of Erlangen-Nuremberg, Erlangen, Germany Abstract The clinical outcome of adoptive T cell transfer-based immunotherapies is often limited due to different escape mechanisms established by tumors in order to evade the hosts’ immune system. The establishment of an immunosuppressive micromilieu by tumor cells along with distinct subsets of tumor-infiltrating lymphocytes is often associated with oxidative stress that can affect antigen-specific memory/effector cytotoxic T cells thereby substantially reducing their frequency and functional activation. Therefore, protection of tumor-reactive cytotoxic T lymphocytes from oxidative stress may enhance the anti-tumor-directed immune response. In order to better define the key pathways/proteins involved in the response to ¨ + + oxidative stress a comparative 2-DE-based proteome analysis of naıve CD45RA and their memory/effector CD45RO T c