conformational altered p53 as an early marker of oxidative stress in alzheimers disease构象改变p53作为氧化应激在阿尔茨海默病的早期标志.pdf

Conformational Altered p53 as an Early Marker of Oxidative Stress in Alzheimer’s Disease 1. 1,2. 3 1 1 Laura Buizza , Giovanna Cenini , Cristina Lanni , Giulia Ferrari-Toninelli , Chiara Prandelli , Stefano 3 3 3 4 4 5 Govoni , Erica Buoso , Marco Racchi , Maria Barcikowska , Maria Styczynska , Aleksandra Szybinska , 2 1 1 David Allan Butterfield , Maurizio Memo , Daniela Uberti * 1 Department of Biomedical Sciences and Biotechnologies, University of Brescia, Brescia, Italy, 2 Sanders-Brown Centre on Aging, University of Kentucky, Lexington, Kentucky, United States of America, 3 Department of Experimental and Applied Pharmacology, University of Pavia, Pavia, Italy, 4 Medical Research Centre Polish Academy of Science, Warszawa, Poland, 5 Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Warszawa, Poland Abstract In order to study oxidative stress in peripheral cells of Alzheimer’s disease (AD) patients, immortalized lymphocytes derived from two peculiar cohorts of patients, referring to early onset AD (EOSAD) and subjects harboured AD related mutation (ADmut), were used. Oxidative stress was evaluated measuring i) the typical oxidative markers, such as HNE Michel adducts, 3 Nitro-Tyrosine residues and protein carbonyl on protein extracts, ii) and the antioxidant capacity, following the enzymatic kinetic of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRD). We found that the signs of oxidative stress, measured as