γ-tocotrienol prevents oxidative stress-induced telomere shortening in human fibroblasts derived from different aged individualsγ-tocotrienol防止氧化应激在人类成纤维细胞端粒缩短来自不同的年龄的人.pdf

ReseaRch PaPeR ReseaRch PaPeR Oxidative Medicine and cellular Longevity, 3:1 35-43; January/February 2010; © 2010 Landes Bioscience γ-Tocotrienol prevents oxidative stress-induced telomere shortening in human fibroblasts derived from different aged individuals 1, 1 1 1 2 1 suzana Makpol, * azrina Zainal abidin, Khalilah sairin, Musalmah Mazlan, Gapor Md. Top and Wan Zurinah Wan Ngah 1Department of Biochemistry; Faculty of Medicine; National University of Malaysia; Kuala Lumpur, Malaysia; 2 Department of Chemistry; Malaysian Palm Oil Board; Bangi, Selangor Malaysia Key words: γ-tocotrienol, telomere, telomerase, fibroblasts, oxidative stress, aging Abbreviations: ALT, alternative lengthening of telomeres; CCM, complete culture media; CO , carbon dioxide; DNA, 2 deoxyribonucleic acid; DIG, digoxigenin; EMEM, eagle’s minimun essential medium; FCS, fetal calf serum; GTT, γ-tocotrienol; HDF, human diploid fibroblast; H O , hydrogen peroxide; IC , inhibitory concentration; IMDM, iscove’s modified dulbecco’s 2 2 50 medium; MF, fibroblasts derived from middle age human; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-carboxymethoxyphenyl 2-(4-sulfophenyl)-2H-tetrazolium; OF, fibroblasts derived from old age human; PCR, polymerase chain reaction; PMS, phenazine methosulphate; ROS, reactive o