senescence of human vocal fold fibroblasts in primary culture人类声襞的衰老成纤维细胞在初级文化.pdf

J. Biomedical Science and Engineering, 2010, 3, 148-159 JBiSE doi:10.4236/jbise.2010.32020 Published Online February 2010 (http://www.SciRP.org/journal/jbise/). Senescence of human vocal fold fibroblasts in primary culture George M. Adams1 1 1,2 , Chet C. Xu , Roger W. Chan 1Otolaryngology, Head and Neck Surgery, University of Texas Southwestern Medical Center, Dallas, USA; 2Biomedical Engineering, University of Texas Southwestern Medical Center, Dallas, USA. Email: roger.chan@ Received 1 October 2009; revised 18 December 2009; accepted 21 December 2009. ABSTRACT thologies. Tissue engineering, or regenerative medicine approaches involve the fabrication of tissue substitutes This study examined the replicative senescence of that can be used to surgically augment, replace, and primary-culture human vocal fold fibroblasts, in terms functionally repair tissue defects in the lamina propria, of changes in gross chromosomal structure with sub- e.g., vocal fold scarring, atrophy, sulculs vocalis, and culturing, and population doubling time (PDT). The tissue deficiencies left with the surgical removal of tu- mRNA expressions of 14 target genes were also ex-