comparative transcriptional and genomic analysis of plasmodium falciparum field isolates比较转录和恶性疟原虫基因组分析现场隔离.pdf

Comparative Transcriptional and Genomic Analysis of Plasmodium falciparum Field Isolates 1 2 2 1 1 2 Margaret J. Mackinnon *, Jinguang Li , Sachel Mok , Moses M. Kortok , Kevin Marsh , Peter R. Preiser , Zbynek Bozdech2 1 KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya, 2 Department of Biological Sciences, Nanyang Technological University, Nanyang, Singapore Abstract Mechanisms for differential regulation of gene expression may underlie much of the phenotypic variation and adaptability of malaria parasites. Here we describe transcriptional variation among culture-adapted field isolates of Plasmodium falciparum, the species responsible for most malarial disease. It was found that genes coding for parasite protein export into the red cell cytosol and onto its surface, and genes coding for sexual stage proteins involved in parasite transmission are up- regulated in field isolates compared with long-term laboratory isolates. Much of this variability was associated with the loss of small or large chromosomal segments, or other forms of gene copy number variation that are prevalent in the P. falciparum genome (copy number variants, CNVs). Expression levels of genes inside these segments were correlated to that of genes outside and adjacent to the segment boundaries, and this association declined with distance from the CNV boundary. This observation could not be explained by copy number variation in these adjacent genes. This suggests a local- acting regulatory role for CNVs in transcription of neighboring genes and helps explain the chromosomal clustering that we observed here. Transcriptional co-regulation of physical clusters of adaptive genes may provide a way for