comparative genomic analysis of pathogenic and probiotic enterococcus faecalis isolates, and their transcriptional responses to growth in human urine比较基因组分析对致病性和益生菌粪肠球菌的分离,以及他们的转录反应在人尿中增长.pdf

Comparative Genomic Analysis of Pathogenic and Probiotic Enterococcus faecalis Isolates, and Their Transcriptional Responses to Growth in Human Urine 1.¤ 1. 2 1 1 Heidi C. Vebø , Margrete Solheim , Lars Snipen , Ingolf F. Nes , Dag A. Brede * 1 Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life ˚ ˚ Sciences, As, Norway, 2 Section for Biostatistics, Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, As, Norway Abstract Urinary tract infection (UTI) is the most common infection caused by enterococci, and Enterococcus faecalis accounts for the majority of enterococcal infections. Although a number of virulence related traits have been established, no comprehensive genomic or transcriptomic studies have been conducted to investigate how to distinguish pathogenic from non-pathogenic E. faecalis in their ability to cause UTI. In order to identify potential genetic traits or gene regulatory features that distinguish pathogenic from non-pathogenic E. faecalis with respect to UTI, we have performed comparative genomic analysis, and investigated growth capacity and transcriptome profiling in human urine in vitro. Six strains of different origins were cultivated and all grew readily in human urine. The three strains chosen for transcriptional analysis showed an overall similar response with respect to energy and nitrogen metabolism, stress mechanism, cell envelop