comparative genomic and transcriptomic characterization of the toxigenic marine dinoflagellate alexandrium ostenfeldii比较基因组和转录组的产毒素的海洋腰鞭毛虫适宜ostenfeldii.pdf

Comparative Genomic and Transcriptomic Characterization of the Toxigenic Marine Dinoflagellate Alexandrium ostenfeldii 1 2 1 ¨ 3,4 5 6 Nina Jaeckisch *, Ines Yang , Sylke Wohlrab , Gernot Glockner , Juergen Kroymann , Heiko Vogel , 1 1 Allan Cembella , Uwe John * ¨ 1 Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany, 2 Medizinische Hochschule Hannover, Institut fur Medizinische Mikrobiologie und Krankenhaushygiene, Hannover, Germany, 3 Berlin Center for Genomics in Biodiversity Research, Berlin, Germany, 4 Institute for Freshwater Ecology and Inland Fisheries, ´ ´ Berlin, Germany, 5 Universite Paris-Sud/CNRS, Laboratoire d’Ecologie, Systematique et Evolution, Orsay, France, 6 Max Planck Institute for Chemical Ecology, Jena, Germany Abstract Many dinoflagellate species are notorious for the toxins they produce and ecological and human health consequences associated with harmful algal blooms (HABs). Dinoflagellates are particularly refractory to genomic analysis due to the enormous genome size, lack of knowledge about their DNA composition and structure, and peculiarities of gene regulation, such as spliced leader (SL) trans-splicing and mRNA transposition mechanisms. Alexandrium ostenfeldii is known to produce macrocyclic imine toxins, described as spirolides. We characterized the genome of A. ostenfeldii using a combination of transcriptomic data and random genomic clone