comparative analysis of viral gene expression programs during poxvirus infection a transcriptional map of the vaccinia and monkeypox genomes比较分析病毒基因表达程序在痘病毒感染牛痘和猴痘的基因组的转录图谱.pdf

Comparative Analysis of Viral Gene Expression Programs during Poxvirus Infection: A Transcriptional Map of the Vaccinia and Monkeypox Genomes 1,2¤ 4 5 6 6 Kathleen H. Rubins *, Lisa E. Hensley , George W. Bell , Chunlin Wang , Elliot J. Lefkowitz , Patrick O. Brown2,3, David A. Relman 1,6,7 1 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, United States of America, 2 Biochemistry, Stanford University School of Medicine, Stanford, California, United States of America, 3 Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, United States of America, 4 US Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, United States of America, 5 Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America, 6 University of Alabama at Birmingham, Birmingham, Alabama, United States of America, 7 Department of Medicine, Stanford University School of Medicine, and Veterans Affairs Palo Alto Health Care System, Palo Alto, California, United States of America Abstract Background: Poxviruses engage in a complex and intricate dialogue with host cells as part of their strategy for replication. However, relatively little molecular detail is available with which to understand the mechanisms behind this dialogue. Methodology/Principal Findings: We designed a specialized microarray that contains probes specific to all predicted ORFs in the Monkeypox Zaire (MPXV) and Vaccinia Western Reserve (VACV) genomes, as well as .18,000 human genes, and used this tool to characterize MPXV and VACV gene expression responses in vitro during the course of primary infection of human monocytes, primary human fibroblasts and He