big genomes facilitate the comparative identification of regulatory elements大基因组促进监管的比较识别元素.pdf

Big Genomes Facilitate the Comparative Identification of Regulatory Elements 1,2. 1,2. 1 1 3 3 Brant K. Peterson , Emily E. Hare , Venky N. Iyer , Steven Storage , Laura Conner , Daniel R. Papaj , 4 4 1,2,5,6,7 Rick Kurashima , Eric Jang , Michael B. Eisen * 1 Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America, 2 Genomics Division, Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, 3 Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona, United States of America, 4 Pacific Basin Agricultural Research Center, United States Department of Agriculture, Hilo, Hawaii, United States of America, 5 Howard Hughes Medical Institute, University of California, Berkeley, California, United States of America, 6 California Institute of Quantitative Biosciences, University of California, Berkeley, California, United States of America, 7 Center for Integrative Genomics, University of California, Berkeley, California, United States of America Abstract The identification of regulatory sequences in animal genomes remains a significant challenge. Comparative genomic methods that use patterns of evolutionary conservation to identify non-coding sequences with regulatory function have yielded many new vertebrate enhancers. However, these methods have not contributed significantly to the identification of regulatory sequences in sequenced invertebrate taxa. We demonstrate here that this differential success, whic