the complete genome sequence of the pathogenic intestinal spirochete brachyspira pilosicoli and comparison with other brachyspira genomes完整的基因组序列的致病性肠道螺旋体brachyspira pilosicoli与其他brachyspira基因组和比较.pdf

The Complete Genome Sequence of the Pathogenic Intestinal Spirochete Brachyspira pilosicoli and Comparison with Other Brachyspira Genomes 1,2 1 3 1 1 Phatthanaphong Wanchanthuek , Matthew I. Bellgard , Tom La , Karon Ryan , Paula Moolhuijzen , 1 1 1 1 1 3 Brett Chapman , Michael Black , David Schibeci , Adam Hunter , Roberto Barrero , Nyree D. Phillips , David J. Hampson3* 1 Centre for Comparative Genomics, Murdoch University, Perth, Western Australia, Australia, 2 Faculty of Informatics, Mahasarakham University, Mahasarakham, Thailand, 3 Animal Research Institute, School of Veterinary and Biomedical Science, Murdoch University, Perth, Western Australia, Australia Abstract Background: The anaerobic spirochete Brachyspira pilosicoli colonizes the large intestine of various species of birds and mammals, including humans. It causes ‘‘intestinal spirochetosis’’, a condition characterized by mild colitis, diarrhea and reduced growth. This study aimed to sequence and analyse the bacterial genome to investigate the genetic basis of its specialized ecology and virulence. Methodology/Principal Findings: The genome of B. pilosicoli 95/1000 was sequenced, assembled and compared with that of the pathogenic Brachyspira hyodysenteriae and a near-complete sequence of Brachyspira murdochii. The B. pilosicoli genome was circular, composed of 2,586,443 bp with a 27.9 mol% G+C content, and encoded 2,338 genes. The three Brachyspira species shared 1,087 genes and showed evidence of extensive genome rearrangements.