the complete sequence of a human parainfluenzavirus 4 genome完整的人类parainfluenzavirus 4基因组序列.pdf

Viruses 2009, 1, 26-41; doi:10.3390/v1010026 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article The Complete Sequence of a Human Parainfluenzavirus 4 Genome 1 2 2 2 2 Carmen Yea , Rose Cheung , Carol Collins , Dena Adachi , John Nishikawa and Raymond Tellier 1,2,3,4,* 1 Program in Genetics and Genome Biology, Research Institute, Hospital for Sick Children, Toronto Ontario, Canada; E-mails: carmen.yea@sickkids.ca (C.Y.) 2 Division of Microbiology, Hospital for Sick Children, Toronto, Ontario, Canada; E-mails: carol.collins@sickkids.ca (C.C.) dena.adachi@sickkids.ca (D.A.), john.nishikawa@sickkids.ca (J.N.) 3 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada 4 Current address: Provincial Laboratory for Public Health (Microbiology), Alberta, and University of Calgary, Calgary, Alberta, Canada * Author to whom correspondence should be addressed; E-mail: r.tellier@provlab.ab.ca; Tel.: +1 403 944 1200; Fax: +1 404 283 0142 Received: 6 April 2009; in revised form: 22 May 2009 / Accepted: 26 May 2009 / Published: 2 June 2009 Abstract: Although the human parainfluenza virus 4 (HPIV4) has been known for a long time, its genome, alone among the human paramyxoviruses, has not been completely sequenced to date. In this study we obtained the first complete genomic sequence of HPIV4 from a clinical isolate named