the complete genome sequence of natrinema sp. j7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements的完整基因组序列natrinema sp,j7-2 haloarchaeon能力增长合成媒体没有氨基酸补充剂.pdf

The Complete Genome Sequence of Natrinema sp. J7-2, a Haloarchaeon Capable of Growth on Synthetic Media without Amino Acid Supplements 1. 2. 1 2 2 1 1 1 Jie Feng , Bin Liu , Ziqian Zhang , Yan Ren , Yang Li , Fei Gan , Yuping Huang , Xiangdong Chen , 1 2,3 1 1 Ping Shen , Lei Wang , Bing Tang *, Xiao-Feng Tang * 1 College of Life Sciences, Wuhan University, Wuhan, Hubei, People’s Republic of China, 2 TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, People’s Republic of China, 3 The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, People’s Republic of China Abstract Natrinema sp. J7-2 is an extreme haloarchaeon capable of growing on synthetic media without amino acid supplements. Here we report the complete genome sequence of Natrinema sp. J7-2 which is composed of a 3,697,626-bp chromosome and a 95,989-bp plasmid pJ7-I. This is the first complete genome sequence of a member of the genus Natrinema. We demonstrate that Natrinema sp. J7-2 can use gluconate, glycerol, or acetate as the sole carbon source and that its genome encodes complete metabolic pathways for assimilating these substrates. The biosynthetic pathways for all 20 amino acids have been reconstructed, and we discuss a possible evolutionary relationship between the haloarchaeal arginine synthetic pathway and the bacterial lysine synthetic pathway. The genome harbors the genes for assimilation of ammonium and nitrite, but not nitrate, and has a denitrification pathway to reduce nitrite to N2O. Comparative genomic analysis suggests that most sequenced h