comparative bacterial proteomics analysis of the core genome concept基因组比较细菌蛋白质组学分析的核心概念.pdf

Comparative Bacterial Proteomics: Analysis of the Core Genome Concept 1 2 1 1 3 1 1 Stephen J. Callister , Lee Ann McCue , Joshua E. Turse , Matthew E. Monroe , Kenneth J. Auberry , Richard D. Smith , Joshua N. Adkins *, Mary S. Lipton1* 1 Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 2 Computational Sciences and Mathematics Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 3 Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America While comparative bacterial genomic studies commonly predict a set of genes indicative of common ancestry, experimental validation of the existence of this core genome requires extensive measurement and is typically not undertaken. Enabled by an extensive proteome database developed over six years, we have experimentally verified the expression of proteins predicted from genomic ortholog comparisons among 17 environmental and pathogenic bacteria. More exclusive relationships were observed among the expressed protein content of phenotypically related bacteria, which is indicative of the specific lifestyles associated with these organisms. Although genomic studies can establish relative orthologous relationships among a set of bacteria and propose a set of ancestral genes, our proteomics study establishes expressed lifestyle differences among conserved genes and proposes a set of expressed ancestral traits. Citation: Callister SJ, McCue LA, Turse JE, Monroe ME, Auberry KJ, et al (2008) Comparative Bacterial Proteomics: Analysis of the Core Genome Concept. PLoS ONE 3(2): e1542. doi:10.1371/journal.pone.0001