annotation of two large contiguous regions from the haemonchus contortus genome using rna-seq and comparative analysis with caenorhabditis elegans注释的两大区域之中haemonchus contortus使用rna-seq和比较分析线虫基因组.pdf

Annotation of Two Large Contiguous Regions from the Haemonchus contortus Genome Using RNA-seq and Comparative Analysis with Caenorhabditis elegans 1,2 1 1 2 3 2 Roz Laing , Martin Hunt , Anna V. Protasio , Gary Saunders , Karen Mungall , Steven Laing , Frank 4 1 5 1 6 Jackson , Michael Quail , Robin Beech , Matthew Berriman , John S. Gilleard * 1 Welcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom, 2 Faculty of Veterinary Medicine, University of Glasgow, Glasgow, Strathclyde, United Kingdom, 3 Genome Sciences Centre, BC Cancer Agency, Vancouver, British Columbia, Canada, 4 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, United Kingdom, 5 Institute of Parasitology, McGill University, Ste Anne de Bellevue, Quebec, Canada, 6 Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada Abstract The genomes of numerous parasitic nematodes are currently being sequenced, but their complexity and size, together with high levels of intra-specific sequence variation and a lack of reference genomes, makes their assembly and annotation a challenging task. Haemonchus contortus is an economically significant parasite of livestock that is widely used for basic research as well as for vaccine development and drug discovery. It is one of many medically and economically important parasites within the strongylid nematode group. This group of parasites has the closest phylogenetic relationship with the model organism Caenorhabditis elegans, making comparative analysis a potentially powerful tool for genome annotation and functional studie