the gene expression analysis of blood reveals s100a11 and aqp9 as potential biomarkers of infective endocarditis血液的基因表达分析显示s100a11和aqp9感染性心内膜炎的潜在生物标志物.pdf

The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective Endocarditis 1,2 2 2 2 2 1,2 Franck Thuny , Julien Textoris , Amira Ben Amara , Adil El Filali , Christian Capo , Gilbert Habib , 2 2 Didier Raoult , Jean-Louis Mege * ´ ˆ ´ 1 Departement de Cardiologie, Hopital de la Timone, Aix-Marseille University, Marseille, France, 2 Unite de Recherche sur les Maladies Infectieuses Transmissibles et ´ Emergentes (URMITE), Centre National de la Recherche Scientifique (CNRS) Unite Mixte de Recherche 6236, Aix-Marseille University, Marseille, France Abstract Background: The diagnostic and prognostic assessments of infective endocarditis (IE) are challenging. To investigate the host response during IE and to identify potential biomarkers, we determined the circulating gene expression profile using whole genome microarray analysis. Methods and Results: A transcriptomic case-control study was performed on blood samples from patients with native valve IE (n = 39), excluded IE after an initial suspicion (n = 10) at patient’s admission, and age-matched healthy controls (n = 10). Whole genome microarray analysis showed that patients with IE exhibited a specific transcriptional program with a predominance of gene categories associated with cell activation as well as innate immune and inflammatory responses. Quantitative real-time RT-PCR performed on a selection of highly modu