comparative (meta)genomic analysis and ecological profiling of human gut-specific bacteriophage φb124-14比较基因组分析(元)和生态分析人类gut-specific噬菌体φb124-14.pdf

Comparative (Meta)genomic Analysis and Ecological Profiling of Human Gut-Specific Bacteriophage wB124-14 1 2 1 2¤ 3 4 Lesley A. Ogilvie , Jonathan Caplin , Cinzia Dedi , David Diston , Elizabeth Cheek , Lucas Bowler , 2 2 1 Huw Taylor , James Ebdon , Brian V. Jones * 1 Centre for Biomedical and Health Science Research, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, United Kingdom, 2 School of Environment and Technology, University of Brighton, Brighton, United Kingdom, 3 School of Computing, Engineering and Mathematics, University of Brighton, Brighton, United Kingdom, 4 Sussex Proteomics Centre, University of Sussex, Brighton, United Kingdom Abstract Bacteriophage associated with the human gut microbiome are likely to have an important impact on community structure and function, and provide a wealth of biotechnological opportunities. Despite this, knowledge of the ecology and composition of bacteriophage in the gut bacterial community remains poor, with few well characterized gut-associated phage genomes currently available. Here we describe the identification and in-depth (meta)genomic, proteomic, and ecological analysis of a human gut-specific bacteriophage (designated wB124-14). In doing so we illuminate a fraction of the biological dark matter extant in this ecosystem and its surrounding eco-genomic landscape, identifying a novel and uncharted bacteriophage gene-space in this community. wB124-14 infects only a subset of closely related gut-associated Bacteroides fragilis strains, and the circular genome encodes f