surveillance and genome analysis of human bocavirus in patients with respiratory infection in guangzhou, china监测和基因组分析人类bocavirus呼吸道感染患者在广州,中国.pdf

Surveillance and Genome Analysis of Human Bocavirus in Patients with Respiratory Infection in Guangzhou, China Lin Xu1,2,3., Xia He1,2,3., Ding-mei Zhang1,2,3., Fa-shen Feng1,2,3, Zhu Wang1,2,3, Lin-lin Guan1,2,3, Jue- 1,2,3 4 3,5 3,5 1,2,3 1,2,3 heng Wu , Rong Zhou , Bo-jian Zheng , Kwok-yung Yuen , Meng-feng Li , Kai-yuan Cao * 1 Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of China, 2 Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-Sen University, Guangzhou, People’s Republic of China, 3 Sun Yat-sen University – University of Hong Kong Joint Laboratory of Infectious Disease Surveillance, Sun Yat-sen University, Guangzhou, People’s Republic of China, 4 State Key Laboratory of Respiratory Diseases, Guangzhou Medical University, Guangzhou, People’s Republic of China, 5 Department of Microbiology, University of Hong Kong, Hong Kong SAR, China Abstract Human bocavirus (HBoV) is a novel parvovirus associated with respiratory tract diseases and gastrointestinal illness in adult and pediatric patients throughout the world. To investigate the epidemiological and genetic variation of HBoV in Guangzhou, South China, we screened 3460 throat swab samples from 1686 children and 1774 adults with acute respiratory infection symptoms for HBoV between March 2010 and February 2011, and analyzed the complete genome sequence of 2 HBoV strains. Specimens were screened for HBoV by real-time PCR and other 6 common respiratory viruses by RT-PCR or PCR. HBoV was detected in 58 (1.68%) out of 3460 samples, mostly from pediatric patients (52/58) and inpatient children (