comparative genomic analysis of drosophila melanogaster and vector mosquito developmental genes黑腹果蝇的比较基因组分析和向量蚊子发育的基因.pdf

Comparative Genomic Analysis of Drosophila melanogaster and Vector Mosquito Developmental Genes 1 2 1 1 2 Susanta K. Behura , Morgan Haugen , Ellen Flannery , Joseph Sarro , Charles R. Tessier , David W. Severson1,2, Molly Duman-Scheel1,2* 1 Department of Biological Sciences and Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, United States of America, 2 Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana, United States of America Abstract Genome sequencing projects have presented the opportunity for analysis of developmental genes in three vector mosquito species: Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae. A comparative genomic analysis of developmental genes in Drosophila melanogaster and these three important vectors of human disease was performed in this investigation. While the study was comprehensive, special emphasis centered on genes that 1) are components of developmental signaling pathways, 2) regulate fundamental developmental processes, 3) are critical for the development of tissues of vector importance, 4) function in developmental processes known to have diverged within insects, and 5) encode microRNAs (miRNAs) that regulate developmental transcripts in Drosophila. While most fruit fly developmental genes are conserved in the three vector mosquito species, several genes known to be critical for Drosophila development were not identified in one or more mosquito genomes. In other cases, mosquito lineage-specific gene gains with respect to D. melanogaster were noted. Sequence analyses also revealed that numerous repetitive