a baculovirus immediate-early gene, ie1, promoter drives efficient expression of a transgene in both drosophila melanogaster and bombyx mori杆状病毒基因,即早期ie1,启动子驱动的高效表达黑腹果蝇和家蚕转基因.pdf

A Baculovirus Immediate-Early Gene, ie1, Promoter Drives Efficient Expression of a Transgene in Both Drosophila melanogaster and Bombyx mori 1,2 1 3 1 1 Mika Masumoto , Takahiro Ohde , Kunihiro Shiomi , Toshinobu Yaginuma , Teruyuki Niimi * 1 Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan, 2 Division of Biology, College of Liberal Arts and Sciences, Kitasato University, Sagamihara, Kanagawa, Japan, 3 Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan Abstract Many promoters have been used to drive expression of heterologous transgenes in insects. One major obstacle in the study of non-model insects is the dearth of useful promoters for analysis of gene function. Here, we investigated whether the promoter of the immediate-early gene, ie1, from the Bombyx mori nucleopolyhedrovirus (BmNPV) could be used to drive efficient transgene expression in a wide variety of insects. We used a piggyBac-based vector with a 3xP3-DsRed transformation marker to generate a reporter construct; this construct was used to determine the expression patterns driven by the BmNPV ie1 promoter; we performed a detailed investigation of the promoter in transgene expression pattern in Drosophila melanogaster and in B. mori. Drosophila and Bombyx belong to different insect orders (Diptera and Lepidoptera, respectively); however, and to our surprise, ie1 promoter-driven expression was evident in several tissues (e.g., prothoracic gland, midgut, and tracheole) in both insects. Furthermore, in both species, the ie1 promoter drove expression of the reporter gene from a relatively early embryonic stage, an