buffering of segmental and chromosomal aneuploidies in drosophila melanogaster缓冲段和染色体非整倍性的黑腹果蝇.pdf

Buffering of Segmental and Chromosomal Aneuploidies in Drosophila melanogaster 1,2 1 1 ´ 2,3,4 1 Per Stenberg , Lina E. Lundberg , Anna-Mia Johansson , Patrik Ryden , Malin J. Svensson , Jan Larsson1* ˚ ˚ ˚ ˚ 1 Department of Molecular Biology, Umea University, Umea, Sweden, 2 Computational Life Science Cluster (CLiC), Umea University, Umea, Sweden, 3 Department of ˚ ˚ ˚ ˚ Mathematics and Mathematical Statistics, Umea University, Umea, Sweden, 4 Department of Statistics, Umea University, Umea, Sweden Abstract Chromosomal instability, which involves the deletion and duplication of chromosomes or chromosome parts, is a common feature of cancers, and deficiency screens are commonly used to detect genes involved in various biological pathways. However, despite their importance, the effects of deficiencies, duplications, and chromosome losses on the regulation of whole chromosomes and large chromosome domains are largely unknown. Therefore, to explore these effects, we examined expression patterns of genes in several Drosophila deficiency hemizygotes and a duplication hemizygote using microarrays. The results indicate that genes expressed in deficiency hemizygotes are significantly buffered, and that the buffering effect is general rather than being mainly mediated by feedback regulation of individual genes. In addition, differentially expressed gene