the developmental expression dynamics of drosophila melanogaster transcription factors黑腹果蝇的发育表达动态转录因子.pdf

Adryan and Teichmann Genome Biology 2010, 11:R40 /2010/11/4/R40 R E S E A R C H Open Access Research The developmental expression dynamics of Drosophila melanogaster transcription factors 1,2 1 Boris Adryan* and Sarah A Teichmann* Transcription factor cotranscription factors during embryogenesis innisms of transcriptional control.High levels of combinatorial complexity ofDrosophila melanogastermbinatorial complexityshed light on mecha- Abstract Background: Site-specific transcription factors (TFs) are coordinators of developmental and physiological gene expression programs. Their binding to cis-regulatory modules of target genes mediates the precise cell- and context- specific activation and repression of genes. The expression of TFs should therefore reflect the core expression program of each cell. Results: We studied the expression dynamics of about 750 TFs using the available genomics resources in Drosophila melanogaster. We find that 95% of these TFs are expressed at some point during embryonic development, with a peak roughly between 10 and 12 hours after egg laying, the core stages of organogenesis. We address the differential utilization of DNA-binding domains in different developmental programs systematically in a spatio-temporal context, and show that the zinc finger class of TFs is predominantly early expressed, while Homeobox TFs exhibit later expression in embryogenesis. Conclusions: Previous work, dissecting cis-regulatory modules during Drosophila development, suggests that TFs are deployed in groups acting in a cooperative manner. In contrast, we find that there is rapid exchange of co-expressed partners amongst the fly TFs, at rates similar to the genome-wide dynamics of co-expression clusters. This suggests there may also b