whole-genome analysis reveals that active heat shock factor binding sites are mostly associated with non-heat shock genes in drosophila melanogaster全基因组分析表明,主动热休克因子结合位点大多与不冲击基因在果蝇.pdf

Whole-Genome Analysis Reveals That Active Heat Shock Factor Binding Sites Are Mostly Associated with Non- Heat Shock Genes in Drosophila melanogaster 1 2,3 1 4,5 1 Sarah E. Gonsalves , Alan M. Moses , Zak Razak , Francois Robert , J. Timothy Westwood * 1 Department of Cell and Systems Biology, University of Toronto, Mississauga, Canada, 2 Department of Cell and Systems Biology, University of Toronto, Toronto, Canada, ´ ´ 3 Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada, 4 Institut de Recherches Cliniques de Montreal, Montreal, Canada, ´ ´ ´ ´ ´ ´ ´ 5 Departement de Medecine, Faculte de Medecine, Universite de Montreal, Montreal, Canada Abstract During heat shock (HS) and other stresses, HS gene transcription in eukaryotes is up-regulated by the transcription factor heat shock factor (HSF). While the identities of the major HS genes have been known for more than 30 years, it has been suspected that HSF binds to numerous other genes and potentially regulates their transcription. In this study, we have used a chromatin immunoprecipitation and microarray (ChIP-chip) approach to identify 434 regions in the Drosophila genome that are bound by HSF. We have also performed a transcript analysis of heat shocked Kc167 cells and third instar larvae and compared them to HSF binding sites. The heat-induced transcription profiles were quite different between cells and larvae and surprisingly only about 10% of the genes associated with HSF binding sites show changed transcri