cytotoxic cembranes from indonesian specimens of the soft coral nephthea sp.细胞毒性cembranes从印尼标本的软珊瑚nephthea sp。.pdf

Mar. Drugs 2010, 8, 2142-2152; doi:10.3390/md8072142 OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Cytotoxic Cembranes from Indonesian Specimens of the Soft Coral Nephthea sp. Hedi Indra Januar 1,2, Ekowati Chasanah 1,2, Cherie A. Motti 2, Dianne M. Tapiolas 2, Catherine H. Liptrot 2,† and Anthony D. Wright 2,3,* 1 Indonesia Research Center for Marine and Fisheries Product Processing and Biotechnology, Jl. KS Tubun Petamburan VI, Jakarta 10260, Indonesia; E-Mails: idjanuar@ (H.I.J.); ekowati_ch@ (E.C.) 2 Australian Institute of Marine Science, PMB No. 3, Townsville MC, Townsville, 4810, Australia; E-Mails: c.motti@.au (C.A.M.); d.tapiolas@.au (D.M.T.) 3 University of Hawaii, College of Pharmacy, 34 Rainbow Drive, Hilo, HI 96720, USA † Current address: James Cook University, Townsville, QLD 4811, Australia; E-Mail: catherine.liptrot@.au. * Author to whom correspondence should be addressed; E-Mail: adwright@; Tel.: +1-808-933-2866; Fax: +1-808-443-5903. Received: 19 June 2010; in revised form: 6 July 2010 / Accepted: 9 July 2010 / Published: 13 July 2010 Abstract: Methanol extracts of two specimens of the soft coral Nephthea sp. collected from the Seribu Islands, Indonesia, were active in an anticancer bioassay. One new (1) and four known diterpenes (2–5) based on the cembrane carbon skeleton were isolated from these extracts, as was arachidonic acid (8). The structures of all compounds were elucidated using NMR, including 1,1-ADEQUATE and 1D