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bio-guided isolation of the cytotoxic terpenoids from the roots of euphorbia kansui against human normal cell lines l-o2 and ges-1bio-guided孤立的细胞毒性萜类化合物的根源甘遂对人类正常细胞株l-o2和ges-1.pdf

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Int. J. Mol. Sci. 2012, 13, 11247-11259; doi:10.3390/ijms130911247 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Bio-Guided Isolation of the Cytotoxic Terpenoids from the Roots of Euphorbia kansui against Human Normal Cell Lines L-O2 and GES-1 Li Zhang, Lan Gao, Zhengjun Li, Xiaojing Yan, Yanjing Yang, Yuping Tang *, Yudan Cao and Anwei Ding * Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China; E-Mails: zhangli@ (L.Z.); gaolan1000@126.com (L.G.); lizhengjun404@126.com (Z.L.); yanxiaojing963@163.com (X.Y.); candy-jing@ (Y.Y.); raindc@163.com (Y.C.) * Authors to whom correspondence should be addressed; E-Mails: yupingtang@ (Y.T.); awding105@163.com (A.D.); Tel./Fax: +86-25-8581-1916 (Y.T.); +86-25-8581-1523(A.D.). Received: 26 July 2012; in revised form: 27 August 2012 / Accepted: 29 August 2012 / Published: 10 September 2012 Abstract: The dried roots of Euphorbia kansui (kansui) have been used for centuries in China as a herbal medicine for edema, ascites, and asthma. The 95% ethanol extract showed a significant inhibition of cell proliferation against human normal cell lines L-O2 and GES-1. Bioassay-guided separation of the 95% ethanol extract from the roots of 1 E. kansui led to the
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