the bioassay-guided isolation of growth inhibitors of adult t-cell leukemia (atl), from the jamaican plant hyptis verticillata, and nmr characterization of hyptoside成人t细胞白血病的获得孤立生长抑制剂(atl),从牙买加植物hyptis verticillata,和hyptoside nmr表征.pdf

Molecules 2012, 17, 9931-9938; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article The Bioassay-Guided Isolation of Growth Inhibitors of Adult T-Cell Leukemia (ATL), from the Jamaican Plant Hyptis verticillata, and NMR Characterization of Hyptoside Toshiyuki Hamada 1,*, Yohann White 2, Mitsuyoshi Nakashima 1, Yusuke Oiso 1, 3 1 1 2 Masaki J. Fujita , Hiroaki Okamura , Tetsuo Iwagawa and Naomichi Arima 1 Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, Japan 2 Division of Hematology and Immunology, Center for Chronic Viral Diseases, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan 3 Priority Organization for Innovation and Excellence, Kumamoto University, 5-1 Oe-Honmachi, Kumamoto 860-8555, Japan * Author to whom correspondence should be addressed; E-Mail: thamada@sci.kagoshima-u.ac.jp; Tel.: +81-99-285-8114; Fax: +81-99-285-8117. Received: 12 June 2012; in revised form: 7 August 2012 / Accepted: 8 August 2012 / Published: 17 August 2012 Abstract: Through bioassay-guided isolation, five compounds with growth inhibitory activity on S1T, an adult T-cell leukemia (ATL) cell line, were isolated from the crude methanol extract of the aerial parts of Hyptis verticillata . Keywords: ATL; bioassay-guided isolation; S1T; growth inhibitor; lignan; NMR; struct