the growth suppressing effects of girinimbine on hepg2 involve induction of apoptosis and cell cycle arrest生长抑制girinimbine对hepg2的影响涉及诱导细胞凋亡和细胞周期阻滞.pdf

Molecules 2011, 16, 7155-7170; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article The Growth Suppressing Effects of Girinimbine on Hepg2 Involve Induction of Apoptosis and Cell Cycle Arrest Suvitha Syam 1, Ahmad Bustamam Abdul 1,*, Mohd. Aspollah Sukari 2, Syam Mohan 3, 4 2 Siddig Ibrahim Abdelwahab and Tang Sook Wah 1 UPM-Makna Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 3 Centre for Natural Products and Drug Discovery (CENAR), Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia 4 Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia * Author to whom correspondence should be addressed; E-Mail: suvithasyam@; Tel.: 006017-3195643; Fax: 00603 Received: 18 May 2011; in revised form: 6 July 2011 / Accepted: 3 August 2011 / Published: 23 August 2011 Abstract: Murraya koenigii is an edible herb widely used in folk medicine. Here we report that girinimbine, a carbazole alkaloid isolated from this plant, inhibited the growth and induced apoptosis in human hepatocellular carcinoma, HepG2 cells. The MTT and LDH assay results showed that girinimbine decreased cell viability and increased cytotoxicity in a dose-and time-dep