cytotoxicity of the ascidian cystodytes dellechiajei against tumor cells and study of the involvement of associated microbiota in the production of cytotoxic compounds细胞毒性的海鞘类cystodytes dellechiajei对肿瘤细胞和研究相关的微生物群的参与生产的细胞毒性的化合物.pdf

Mar. Drugs 2007, 5, 52-70 Marine Drugs ISSN 1660-3397 © 2007 by MDPI /marinedrugs Full Original Paper Cytotoxicity of the Ascidian Cystodytes dellechiajei Against Tumor Cells and Study of the Involvement of Associated Microbiota in the Production of Cytotoxic Compounds Manuel Martínez-García 1,*, Marta Díaz-Valdés 2, Alfonso Ramos-Esplá 2, Nélida Salvador 3, Patricia Lopez 1, Eduardo Larriba 1 and Josefa Antón 1 1 División de Microbiología, Universidad de Alicante, 03080 Alicante, Spain 2 Departamento de Ciencias del Mar y Biología Aplicada, Universidad de Alicante, 03080 Alicante, Spain 3 Instituto Biomar, S. A. Pol. Ind. Edificio CEI 2.02, 24231 Onzonilla, León, Spain * Author to whom correspondence should be addressed; E-mail: m.martinez@ua.es Received: 18 June 2007 / Accepted: 17 July 2007 / Published: 19 July 2007 Abstract: Many cytotoxic compounds of therapeutic interest have been isolated from marine invertebrates, and some of them have been reported to be of microbial origin. Pyridoacridine alkaloids are the main compounds extracted from the ascidian Cystodytes dellechiajei. Here we describe the in vitro antiproliferative activity against different tumor cell lines of the ascidian extracts and provide some insights on the role of the microbial community associated with the tunicate in the production of these compounds. C. dellechiajei extracts showed remarkably high antiproliferative activity (IC5