cytotoxic aaptamines from malaysian aaptos aaptos细胞毒性aaptamines从马来西亚aaptos aaptos.pdf

Mar. Drugs 2009, 7, 1-8; doi:10.3390/md7010001 OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Cytotoxic Aaptamines from Malaysian Aaptos aaptos Khozirah Shaari 1,*, Kee Cheng Ling 1, Zalilawati Mat Rashid 2, Tan Pei Jean 1 Faridah Abas 1, 1 1 1 2 Salahudin Mohd. Raof , Zurina Zainal , Nordin Hj. Lajis , Habsah Mohamad and Abdul 3 Manaf Ali 1 Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2 Department of Chemical Sciences, Faculty of Science and Technology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia. 3 Faculty of Biotechnology, Universiti Darul Iman, Kuala Terengganu, Terengganu, Malaysia. * Author to whom correspondence should be addressed. Email: khozirah@.my Received: 10 November; in revised: 12 December 2008/ Accepted: 12 December 2008/ Published: 28 December 2008 Abstract: In a preliminary screen, Aaptos aaptos showed significant cytotoxic activity towards a panel of cell lines and was thus subjected to bioassay-guided isolation of the bioactive constituents. In addition to the known aaptamine, two new derivatives of the alkaloid were isolated from the bioactive chloroform fraction of the crude methanolic extract. Detailed analysis by NMR and mass spectroscopy enabled their iden