steroids from the soft coral sinularia crassa类固醇的软珊瑚sinularia菌.pdf

Mar. Drugs 2012, 10, 439-450; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Steroids from the Soft Coral Sinularia crassa Chih-Hua Chao 1,2, Kuei-Ju Chou 3, Chiung-Yao Huang 3, Zhi-Hong Wen 3,4, Chi-Hsin Hsu 3,4, Yang-Chang Wu 5, Chang-Feng Dai 6 and Jyh-Horng Sheu 3,4,* 1 Chinese Medicinal Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan; E-Mail: chaochihhua@ 2 China Medical University, Taichung, 40402, Taiwan 3 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; E-Mails: jzusmile@ (K.-J.C.); betty8575@.tw (C.-Y.H.); wzh@.tw (Z.-H.W.); hsuch@.tw (C.-H.H.) 4 Asian Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan 5 Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; E-Mail: yachwu@.tw 6 Institute of Oceanography, National Taiwan University, Taipei, 10617, Taiwan; E-Mail: corallab@.tw * Author to whom correspondence should be addressed; E-Mail: sheu@.tw; Tel.: +886-7-5252000 (ext. 5030); Fax: +886-7-5255020. Received: 9 January 2012; in revised form: 7 February 2012 / Accepted: 9 February 2012 / Published: 16 February 2012 Abstract: One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A–D (2–5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher’s method. The absolut