培养局的纯培养（Pure culture of culture board）.doc

培养局的纯培养（Pure culture of culture board） Experiment six pure culture of microorganisms First, the purpose and requirements 1. learn and master various aseptic manipulation techniques, and use this technique for microbial dilution separation, streak separation and inoculation. 2. microorganisms were separated by plate marking and dilution. 3. to understand the culture conditions and culture time of four kinds of microorganisms. 3. understand the universality of microorganisms and understand the importance of aseptic manipulation. Two 、 experimental principle Pure culture technology is the most basic operation technique in the laboratory, the experiment purpose, medium types and different containers, different inoculation methods used, are good for microorganism, therefore, usually should be carried out after inoculation disinfection asepsis room or vaccination box in the air, at the same time due to different methods of inoculation using different tools, often with. Isolation and purification of microorganisms: isolation of microorganisms with special functions, fine strains and purification of contaminated bacteria. Pure breed isolation is one of the most important basic techniques in microbiology. Isolation: the method of pure culture of a single strain from a promiscuous microbial population. Purified (pure culture): a cell or spore of a bacterium or a culture is produced by the division and propagation of a single cell. The methods of isolation and purification of microorganisms are common: 1. simple single cell picking method Single cell picking method can directly get the pure culture. It requires special micromanipulator to separate single cells, or to directly separate spores under ordinary microscopy at low magnification. 2. plate separation method There are two kinds of dilution method: pouring method and coating method. The bacteria are separated by other miscellaneous bacteria or mixed bacteria suspension. The method is easy to operate and widely used in th