17-第五章 培养基及设备的灭菌（Chapter 17 the sterilization of the culture medium and equipment）.doc

17-第五章 培养基及设备的灭菌（Chapter 17 the sterilization of the culture medium and equipment） Chapter 5 sterilization of medium and equipment The purpose, requirements and methods of culture medium sterilization A, definitions, 1. Definition of culture medium sterilization It refers to a bacterial nutrient or spore that kills a living capacity from a culture medium, or removes it from it. The sterilization of the liquid medium of industrial scale is more common than the removal of mixed bacteria. 2. The difference between sterilization and sterilization Sterilization: kill or remove all microorganisms in the environment by physical or chemical methods, including nutrient cells, bacterial spores and spores Disinfection: the use of physical or chemical methods to kill the pathogenic microorganisms in materials, containers and vessels. The purpose of sterilization of culture medium 1. The effects of mixed fungi in the fermentation process: The production bacteria and the bacteria grow at the same time, and the production capacity of the production bacteria is lost. In the course of continuous fermentation, the growth rate of the heterobacteria can sometimes grow faster than the production of the bacteria, and the result is that the yeast can be used in the yeast. Heterobacteria and their resulting substances make it difficult to extract and refine The result of the degradation of the heterobacteria; The bacteria will contaminate the final product, and the bacteria will contaminate the final product. When fermentation, such as contaminating bacteriophages, the bacteria can be produced by bacteria. 2. Specific industrial measures include: (1) the medium and equipment used must be sterilized; (2) the air used in aerobic culture should be treated with sterilization; (3) the equipment should be strict and the fermentation tank should maintain the positive pressure environment; (4) the materials to be added in the cultivation process should be sterilized; (5) use uncontaminated pure se