牛血清在细胞培养基中的主要功能(The main function of bovine serum in cell culture medium).doc
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牛血清在细胞培养基中的主要功能(The main function of bovine serum in cell culture medium)
Bovine serum is the largest natural medium used in cell culture. It contains abundant nutrients and essential nutrients for cell growth.
1. Provide the hormone that maintains the growth of the cell index, which has no or small amounts of nutrients in the base medium, as well as the main low-molecular nutrients.
2. Provide binding proteins that can recognize vitamins, lipids, metals and other hormones, and can combine or adjust the material vitality they combine.
In some cases, a combination of protein and toxic metal and hot raw material can be used to detoxify.
It is the source of the factors needed for the cell wall and the spread of the plastic substrate.
5. Effect of acid alkalinity buffer.
6. Provide a protease inhibitor, which causes the remaining trypsin to be lost while the cell is passed, to protect the cells from injury.
The main components of bovine serum
Serum is a very complex mixture, and although most of its components are known, some are not clear, and the serum composition and content are constant
The sex, age, physiological condition and nutritional condition of the donor were different.
Protein is the main ingredient in cow serum. In addition to the fact that you can carry metal ions, fatty acids, and your own hormone protein, white protein, globulin.
Fiber adhesion cells promote cell adhesion;
Alpha 2 macroglobulin inhibits the action of trypsin; The fetal bovine serum contains fetal globulins that promote cell adhesion; Transferrotein can combine iron ions to reduce toxicity
And theyre used by cells.
2. Peptides: thrombocytopenia can promote cell division and is one of the main members of polypeptide family, which is the main pro-cell proliferation factor. fibroblast
Growth factor, epidermal cell growth factor, nerve cell growth factor and so on, the content in serum is very small, but it also has a certain role to cell growth.
Hormones: the role of hormones in cells is mu
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