the cebpd (cebpδ) gene is induced by luteinizing hormones in ovarian theca and interstitial cells but is not essential for mouse ovary functioncebpd(cebpδ)基因在卵巢黄体化激素引起的膜间质细胞,但并不是必不可少的小鼠卵巢的功能.pdf

The Cebpd (C/EBPd) Gene Is Induced by Luteinizing Hormones in Ovarian Theca and Interstitial Cells But Is Not Essential for Mouse Ovary Function 1¤a 2¤b 1 3 2 4 1 A-Mei Huang , Martina Rudelius , Shikha Sharan , Jan M. McAllister , Mark Raffeld , Lane K. Christenson , Esta Sterneck * 1 Center for Cancer Research, National Cancer Institute, Frederick, Maryland, United States of America, 2 Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland, United States of America, 3 Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, United States of America, 4 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, United States of America The CCAAT/enhancer binding protein (CEBP) family of transcription factors includes five genes. In the ovary, both Cebpa and Cebpb are essential for granulosa cell function. In this study we have explored the role of the Cebpd gene in ovarian physiology by expression and functional studies. Here we report that Cebpd (C/EBPd) is expressed in the mouse ovary in a highly restricted temporal and spatial pattern. In response to luteinizing hormone (LH/hCG), CEBPD expression is transiently induced in interstitial cells and in theca cells of follicles from the primary to pre-ovulatory stage, and overlaps in part with expression of the alpha-smooth muscle actin protein. Efficient down-regulation of CEBPD was dependent on a functional Cebpb gene. Proliferating human theca cells in culture also express Cebpd. Cells from patients with polycystic ovarian syndrome (PCOS) exhibited higher Cebpd expression levels. However, deletion of Cebpd in mice had no overt