suppression of inflammation by low-dose methotrexate is mediated by adenosine a2a receptor but not a3 receptor activation in thioglycollate-induced peritonitis抑制炎症的低剂量甲氨蝶呤是由腺苷受体负责但不是a3受体激活thioglycollate-induced腹膜炎.pdf

Available online /content/8/2/R53 Vol 8 No 2 Open Access Research article Suppression of inflammation by low-dose methotrexate is mediated by adenosine A2A receptor but not A3 receptor activation in thioglycollate-induced peritonitis 1,2 2 2 M Carmen Montesinos , Avani Desai and Bruce N Cronstein 1Department of Pharmacology, Universidad de Valencia, Burjassot, Valencia, Spain 2Department of Medicine, New York University School of Medicine, New York, USA Corresponding author: M Carmen Montesinos, m.carmen.montesinos@uv.es Received: 13 Sep 2005 Revisions requested: 26 Oct 2005 Revisions received: 7 Feb 2006 Accepted: 8 Feb 2006 Published: 6 Mar 2006 Arthritis Research Therapy 2006, 8:R53 (doi:10.1186/ar1914) This article is online at: /content/8/2/R53 © 2006 Montesinos et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Prior studies demonstrate that adenosine, acting at one or more in the peritoneal exudates of all mice studied, and reduced the of its receptors, mediates the anti-inflammatory effects of leukocyte accumulation in the wild-type mice and A3 receptor methotrexate in animal models of both acute and chronic knockout mice but not in the A2A receptor knockout mice. inflammation. Both adenosine A2A and A3 receptors contribute Methotrexate reduced exudate levels of TNF-α in