constitutive expression of tnf-related activation-induced cytokine (trance)receptor activating nf-κb ligand (rank)-l by rat plasmacytoid dendritic cells组成型表达的tnf-related activation-induced细胞因子(恍惚)受体激活nf-κb配体(等级)- l鼠血浆树突细胞.pdf

Constitutive Expression of TNF-Related Activation- Induced Cytokine (TRANCE)/Receptor Activating NF-kB Ligand (RANK)-L by Rat Plasmacytoid Dendritic Cells 1,2¤ ´ ˆ 1,2,3 1,2 1,2 Thomas Anjubault , Jerome Martin , Franc¸ois-Xavier Hubert , Camille Chauvin , Dominique 4,5 ´ 1,2,3,5 Heymann , Regis Josien * ´ 1 INSERM UMR 1064, Nantes, France, 2 CHU Nantes, Institut de Transplantation Urologie Nephrologie (ITUN), Nantes, France, 3 CHU Nantes, Laboratoire d’Immunologie, ´ ´ ´ Nantes, France, 4 INSERM UMR 791, Nantes, France, 5 Universite de Nantes, Faculte de Medecine, Nantes, France Abstract Plasmacytoid dendritic cells (pDCs) are a subset of DCs whose major function relies on their capacity to produce large amount of type I IFN upon stimulation via TLR 7 and 9. This function is evolutionary conserved and place pDC in critical position in the innate immune response to virus. Here we show that rat pDC constitutively express TNF-related activation- induced cytokine (TRANCE) also known as Receptor-activating NF-kB ligand (RANKL). TRANCE/RANKL is a member of the TNF superfamily which plays a central role in osteoclastogenesis through its interaction with its receptor RANK. TRANCE/ RANK interaction are also involved in lymphoid organogenesis as well as T cell/DC cross talk. Unlike conventional DC, rat CD4high pDC were shown to constitutively express TRANCE/RANKL both at the mRNA and the surface protein level. TRANCE/ RANKL was also induced on the CD4low subsets of pDC following ac