strong nuclear egfr expression in colorectal carcinomas is associated with cyclin-d1 but not with gene egfr amplification强大的核表皮生长因子受体在大肠癌癌与周期蛋白d1的但不是与表皮生长因子受体基因扩增.pdf
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Dekanić et al. Diagnostic Pathology 2011, 6:108
/content/6/1/108
RESEARCH Open Access
Strong nuclear EGFR expression in colorectal
carcinomas is associated with cyclin-D1 but not
with gene EGFR amplification
1 2 1 1 3
Andrea Dekanić , Renata Dobrila Dintinjan , Ivana Budisavljević , Sanja Pećanić , Marta Žuvić Butorac and
Nives Jonjić1*
Abstract
Background: Prognostic and predictive significance of epidermal growth factor receptor (EGFR) in colorectal
carcinomas (CRCs) is still controversial. The aim of the present study was to explore and correlate membrane and
nuclear EGFR and cyclin-D1 protein expression with EGFR gene status of tumor cells.
Methods: Immunohistochemical and FISH analysis was performed on 135 archival formalin fixed and paraffin
embedded CRCs.
Results: Strong membrane and strong nuclear EGFR staining was detected in 16% and 57% of cases, respectively,
and strong cyclin-D1 expression in 57% samples. Gene EGFR amplification was identified in 5.9% and polysomy in
7.4% of cases, while 87% showed no EGFR gene changes. A statistically significant difference was only found
between tumor grade and expression of membrane EGFR, while nuclear EGFR and cyclin-D1 expression was not
associated with the clinicopathologic characteristics analyzed. Tumor cells displaying gene amplification and strong
protein membrane EGFR expression overlapped, while EGFR gene status showed no correlation with nuclear EGFR
and cyclin-D1. There was no association between membrane EGFR and cyclin-D1, whereas nuclear EGFR expression
was strongly related to cyclin-D1 expression.
Conclusions: Study results revealed heterogeneity among CRCs, which could have a predictive value by
identifying b
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