decreased expression of the arid1a gene is associated with poor prognosis in primary gastric cancer减少arid1a基因的表达与原发性胃癌预后不良有关.pdf

Decreased Expression of the ARID1A Gene Is Associated with Poor Prognosis in Primary Gastric Cancer 1. 1. 1 1,2 1 1 1 Dan-dan Wang , Yi-bing Chen , Ke Pan , Wei Wang , Shi-ping Chen , Ju-gao Chen , Jing-jing Zhao , 1 1 1 1 1 1 1 Lin Lv , Qiu-zhong Pan , Yong-qiang Li , Qi-jing Wang , Li-xi Huang , Miao-la Ke , Jia He , Jian- chuan Xia1* 1 State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, People’s Republic of China, 2 Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, Guangzhou, People’s Republic of China Abstract Background: The ARID1A gene encodes adenine-thymine (AT)-rich interactive domain-containing protein 1A, which participates in chromatin remodeling. ARID1A has been showed to function as a tumor suppressor in various cancer types. In the current study, we investigated the expression and prognosis value of ARID1A in primary gastric cancer. Meanwhile, the biological role of ARID1A was further investigated using cell model in vitro. Methodology/Principal Findings: To investigate the role of ARID1A gene in primary gastric cancer pathogenesis, real-time quantitative PCR and western blotting were used to examine the ARID1A expression in paired cancerous and noncancerous tissues. Results revealed decreased ARID1A mRNA (P = 0.0029) and protein (P = 0.0015) expression in most tumor-bearing tissues compared with the matched adjacent non-tumor tissues, and in gastric cancer cell lines. To further investigate the clinicop